Fig. 3. Hypoxia and Uremia effects on RBC volume. A. Representative histogram of forward scatter of erythrocytes following 24-h exposure of RBC to normoxia (gray area) or hypoxia (black line). B. RBC obtained from healthy subjects (n=10) were treated with different concentrations of IS (0.01, 0.09, 0.17mM) for 24h at 37°C in an incubator with controlled oxygen under normoxic (21% O₂) (white bars) or hypoxic (5% O₂) (black bars) conditions. Control RBC (CON-RBC) were incubated with TRIS-Glc-BSA in normoxia and hypoxia. RBC were analyzed by flow cytometry. **p<0.01; ***p<0.001 between normoxia versus hypoxia in the same group. ##p<0.01; ###p<0.001 between CON-RBC versus IS concentration in the same group (normoxia vs normoxia; hypoxia vs hypoxia).